5ABY

Complex of C. elegans eIF4E-3 with the 4E-binding protein Mextli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-27
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	91.240, 157.580, 55.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.522 - 1.950
R-factor	0.1737
Rwork	0.172
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5abx
RMSD bond length	0.008
RMSD bond angle	1.045
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	58918
<I/σ(I)>	11.8	2
Completeness [%]	100.0	100
Redundancy	8.3	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.1M TRIS, PH=8.0, 0.2M MGCL2, 17% PEG6000