5ABX
Complex of C. elegans eIF4E-3 with the 4E-binding protein Mextli and cap analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.040, 71.040, 85.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.282 - 1.660 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.19380 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.968 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.300 | 1.700 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 26447 | |
| <I/σ(I)> | 24.5 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.1 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1M SODIUM ACETATE, PH=5.0, 0.01M ZNCL2, 18% PEG6000 |
