5ABV
Complex of D. melanogaster eIF4E with the 4E-binding protein Mextli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.900, 82.020, 84.730 |
| Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
| Resolution | 43.960 - 2.130 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.23180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4UE8 CHAIN A |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.190 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Number of reflections | 50587 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 98.2 | 90.5 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1M BISTRIS PH=6.0, 23% PEG3350 |
