5AB6
Crystal structure of Trypanosoma brucei SCP2-thiolase like protein (TbSLP) in complex with acetoacetyl-CoA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.150, 65.972, 156.755 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 29.430 - 1.900 |
| R-factor | 0.24844 |
| Rwork | 0.248 |
| R-free | 0.26422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.101 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.410 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 0.750 |
| Number of reflections | 184266 | |
| <I/σ(I)> | 6.9 | 2.2 |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE (PH 4.6), 2.0 M AMMONIUM SULPHATE |
