5AB4
Crystal structure of Trypanosoma brucei SCP2-thiolase like protein (TbSLP) form-I.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 |
| Unit cell lengths | 67.448, 67.448, 157.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.867 - 1.751 |
| R-factor | 0.2012 |
| Rwork | 0.199 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zbg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.206 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.870 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 1.360 |
| Number of reflections | 80250 | |
| <I/σ(I)> | 15.3 | 1 |
| Completeness [%] | 99.6 | 98.1 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100 MM SODIUM CITRATE (PH 5.5), 10% (W/V) PEG4000, 200 MM SODIUM ACETATE |
