5AA4
Crystal structure of MltF from Pseudomonas aeruginosa in complex with cell-wall tetrapeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.116, 136.774, 138.098 |
| Unit cell angles | 90.00, 92.34, 90.00 |
Refinement procedure
| Resolution | 48.569 - 2.400 |
| R-factor | 0.1881 |
| Rwork | 0.185 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a5x |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.597 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 1.040 |
| Number of reflections | 94338 | |
| <I/σ(I)> | 13.8 | 1.7 |
| Completeness [%] | 98.5 | 99.9 |
| Redundancy | 6.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
