5AA3

Crystal structure of MltF from Pseudomonas aeruginosa in the presence of tetrasaccharide and tetrapeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	150.605, 136.376, 195.394
Unit cell angles	90.00, 111.38, 90.00

Refinement procedure

Resolution	14.994 - 3.200
R-factor	0.2436
Rwork	0.240
R-free	0.30520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a5x
RMSD bond length	0.013
RMSD bond angle	1.682
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.480	3.250
High resolution limit [Å]	3.200	3.200
Rmerge	0.200	1.250
Number of reflections	120807
<I/σ(I)>	4.7	1.3
Completeness [%]	99.6	99.8
Redundancy	6.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1