5A9V
Structure of apo BipA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | I 41 |
| Unit cell lengths | 241.946, 241.946, 241.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.390 - 3.310 |
| R-factor | 0.26512 |
| Rwork | 0.263 |
| R-free | 0.28781 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3E3X 3cb4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.918 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.510 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.180 | |
| Number of reflections | 86230 | |
| <I/σ(I)> | 17.1 | 1.1 |
| Completeness [%] | 96.1 | 90.1 |
| Redundancy | 17.3 | 15.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG3350, 0.2 M POTASSIUM SODIUM TARTRATE TETRAHYDRATE |
