5A8N
Crystal structure of the native form of beta-glucanase SdGluc5_26A from Saccharophagus degradans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 143.341, 143.341, 143.341 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.910 - 2.050 |
| R-factor | 0.15259 |
| Rwork | 0.151 |
| R-free | 0.17997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WWPDB ENTRY 5A8M |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.800 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.090 | 0.780 |
| Number of reflections | 32184 | |
| <I/σ(I)> | 19.4 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.2 M AMMONIUM SULPHATE, 0.1 M SODIUM CACODYLATE BUFFER PH 6.0, 25% (W/V) PEG 8000 |
