5A8I
Crystal structure of the FHA domain of ArnA from Sulfolobus acidocaldarius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 43.570, 43.570, 71.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.809 - 1.750 |
| R-factor | 0.1686 |
| Rwork | 0.168 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ff4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.002 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CCP4 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.810 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.030 | 0.520 |
| Number of reflections | 13490 | |
| <I/σ(I)> | 21.95 | 1.45 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 4.18 | 4.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | RESERVOIR BUFFER: 0.1 M HEPES PH 7.5; 1.5 M LI2SO4 PROTEIN BUFFER: 20 MM TRIS PH 8.0; 100 MM NACL 3.5 MG/ML PROTEIN IN PROTEIN BUFFER MIX RESERVOIR AND PROTEIN 1:1 |
