5A88
Crystal structure of the riboflavin kinase module of FAD synthetase from Corynebacterium ammoniagenes in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 32 |
| Unit cell lengths | 68.664, 68.664, 147.006 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.820 - 2.080 |
| R-factor | 0.16102 |
| Rwork | 0.159 |
| R-free | 0.18869 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.584 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.220 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 46431 | |
| <I/σ(I)> | 11.2 | 3.8 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10-14% PEG 8000, 20% GLYCEROL, 0.1 M MES/NAOH PH 6.5, 200 MM CACL2 |
