5A7V
The GH130 family of mannoside phosphorylases contains glycoside hydrolases that target beta-1,2 mannosidic linkages in Candida mannan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-22 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 75.767, 118.110, 126.586 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.110 - 1.350 |
| R-factor | 0.131 |
| Rwork | 0.130 |
| R-free | 0.14970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qc2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.707 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.580 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 243880 | |
| <I/σ(I)> | 10.7 | 1.8 |
| Completeness [%] | 98.3 | 98.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.2 M AMMONIUM SULFATE |
