5A7D
Tetrameric assembly of LGN with Inscuteable
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 128.190, 212.580, 280.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.581 - 3.400 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a1s |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.533 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.600 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.230 | 1.420 |
| Number of reflections | 105973 | |
| <I/σ(I)> | 12.7 | 2.15 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.9 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 13% PEG 3350, 0.1 M TRIS PH 7.8, 0.2 M K-THYOCIANATE, 2.5% 1-PROPANOL, 2.5% TACSIMATE PH 7.5 |
