5A6P
Heavy metal associated domain of NLR-type immune receptor Pikp1 from rice (Oryza sativa)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 54.650, 54.650, 235.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.330 - 2.100 |
| R-factor | 0.20354 |
| Rwork | 0.202 |
| R-free | 0.22904 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.792 |
| Phasing software | AutoSol (wizard in) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.330 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 1.170 |
| Number of reflections | 12356 | |
| <I/σ(I)> | 32.3 | 4.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 45 | 46.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS CRYSTALLLIZED WITH 0.1M MIB BUFFER, PH 5.0 AND 25% PEG 1500 |
