5A6P

Heavy metal associated domain of NLR-type immune receptor Pikp1 from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-17
Detector	DECTRIS PIXEL
Spacegroup name	P 65 2 2
Unit cell lengths	54.650, 54.650, 235.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.330 - 2.100
R-factor	0.20354
Rwork	0.202
R-free	0.22904
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.016
RMSD bond angle	1.792
Phasing software	AutoSol (wizard in)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.330	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.080	1.170
Number of reflections	12356
<I/σ(I)>	32.3	4.6
Completeness [%]	100.0	99.9
Redundancy	45	46.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN WAS CRYSTALLLIZED WITH 0.1M MIB BUFFER, PH 5.0 AND 25% PEG 1500