5A6J
GH20C, Beta-hexosaminidase from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Detector | RIGAKU R-AXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.840, 136.940, 202.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 113.410 - 1.940 |
| R-factor | 0.20853 |
| Rwork | 0.206 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2epn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.700 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.110 | 0.340 |
| Number of reflections | 184285 | |
| <I/σ(I)> | 9.2 | 3.9 |
| Completeness [%] | 97.4 | 96.9 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.2 | 12% (W/V) PEG 3350, 0.2 M MAGNESIUM CHLORIDE, 0.05 M CAPS, PH 10.2, 1% GLYCEROL |
