5A6C
Concomitant binding of Afadin to LGN and F-actin directs planar spindle orientation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2014-04-05 |
| Detector | PILATUS |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 169.880, 169.880, 169.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.402 - 2.901 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ro2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.400 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.720 |
| Number of reflections | 34817 | |
| <I/σ(I)> | 14.6 | 1.5 |
| Completeness [%] | 96.3 | 95.8 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.25 | 1.5 M AMMONIUM SULPHATE, 0.1 M TRIS-HCL PH 8.25 |
