5A68
Crystal structure of the AtTTM3 product complex with two orthophosphates and manganese ions (form B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.331, 33.811, 71.812 |
| Unit cell angles | 90.00, 94.40, 90.00 |
Refinement procedure
| Resolution | 43.200 - 1.670 |
| R-factor | 0.18117 |
| Rwork | 0.179 |
| R-free | 0.22223 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.436 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.200 | 1.770 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.080 | 0.620 |
| Number of reflections | 23035 | |
| <I/σ(I)> | 9.5 | 1.75 |
| Completeness [%] | 94.4 | 76.8 |
| Redundancy | 4.1 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M BIS TRIS PH 7.0, 20 % PEG3350 |
