5A61
Crystal structure of full-length E. coli ygiF in complex with tripolyphosphate and two manganese ions.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-15 |
| Detector | DECTRIS PILATUS |
| Wavelength(s) | 0.999990, 1.700020 |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.676, 89.676, 125.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.770 - 1.500 |
| R-factor | 0.15242 |
| Rwork | 0.151 |
| R-free | 0.17087 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.186 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.770 | 1.590 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 1.340 |
| Number of reflections | 91340 | |
| <I/σ(I)> | 26.35 | 2.03 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 15.48 | 14.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3,350, 0.2 M NACL |
