5A60
Crystal structure of full-length E. coli ygiF in complex with tripolyphosphate and two magnesium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Collection date | 2014-03-22 |
| Detector | DECTRIS HYBRID PHOTON |
| Spacegroup name | P 65 |
| Unit cell lengths | 89.924, 89.924, 125.577 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.880 - 1.820 |
| R-factor | 0.15253 |
| Rwork | 0.151 |
| R-free | 0.18948 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.880 | 1.930 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.070 | 0.940 |
| Number of reflections | 50673 | |
| <I/σ(I)> | 14.91 | 2.18 |
| Completeness [%] | 98.8 | 96.9 |
| Redundancy | 9.7 | 9.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3,350, 0.2 M NACL |
