5A55
The native structure of GH101 from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESAEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.260, 89.130, 88.570 |
| Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
| Resolution | 89.090 - 1.850 |
| R-factor | 0.14439 |
| Rwork | 0.143 |
| R-free | 0.17879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.467 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.043 | 0.380 |
| Number of reflections | 88792 | |
| <I/σ(I)> | 47.1 | 4.6 |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 6.9 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 1:1 ratio of protein to 25% (w/v) polyethylene glycol (PEG) 1500 |
