5A53

Crystal Structure of the Rpf2-Rrs1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	287
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 43 21 2
Unit cell lengths	72.280, 72.280, 175.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.505 - 2.401
R-factor	0.1977
Rwork	0.196
R-free	0.22580
Structure solution method	SIRAS
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.651
Data reduction software	MOSFLM
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.510	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.100	1.090
Number of reflections	18921
<I/σ(I)>	17.82	1.92
Completeness [%]	99.7	97.5
Redundancy	11.5	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		CRYSTALS WERE OBTAINED IN 0.2M LISO4, 30 % (W/V) POLYETHYLENE GLYCOL 4000 AND 0.1 M TRIS-HCL PH 8.5, WITH A COMPLEX SOLUTION AT 15 MG/ML CONTAINING TRYPSIN. CRYSTALS WERE CRYOPROTECTED USING SUCCESSIVE SOAKING STEPS IN INCREASING CONCENTRATIONS OF ETHYLENE GLYCOL