5A52
The crystal structure of Arabidopsis thaliana CAR1 in complex with one calcium ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.600, 170.300, 53.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.231 - 1.650 |
| R-factor | 0.2146 |
| Rwork | 0.213 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v29 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.247 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.230 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 22034 | |
| <I/σ(I)> | 20.79 | 3.1 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.5 M MGSO4, 0.5 M HEPES PH 7.0 AND 1.6 M LITHIUM SULFATE |
