5A4X
The crystal structure of Arabidopsis thaliana CAR4 in complex with two calcium ions and Zn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.121, 88.645, 110.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.763 - 2.200 |
| R-factor | 0.2224 |
| Rwork | 0.220 |
| R-free | 0.26550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v29 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.101 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.860 |
| Number of reflections | 36649 | |
| <I/σ(I)> | 17.2 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20 TO 25 % OF POLYETHYLENE GLYCOL 6K, 0.1M MES PH 6.0 TO 6.5 0.1 M ZNCL2 |
