5A4R
Crystal structure of a vitamin B12 trafficking protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-04 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.210, 89.530, 64.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.765 - 2.250 |
| R-factor | 0.23 |
| Rwork | 0.229 |
| R-free | 0.25540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.760 | 2.310 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.020 | 0.600 |
| Number of reflections | 10530 | |
| <I/σ(I)> | 31.6 | 2.6 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 4.9 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22% POLYACRYLIC ACID 5100, 0.02M MAGNESIUM CHLORIDE, 0.1M HEPES PH 7.5 |
