5A4N
Crystal structure of BPSL1147, a PC4 homolog from Burkholderia pseudomallei K96243 (tetragonal crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 56.527, 56.527, 142.524 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.530 - 1.960 |
| R-factor | 0.21373 |
| Rwork | 0.210 |
| R-free | 0.25159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pcf CHAINS A AND B WITH HELICAL REGIONS DELETED |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.652 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.500 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.080 | 1.180 |
| Number of reflections | 17393 | |
| <I/σ(I)> | 21.4 | 2.1 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 10.4 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 295 | 20 MM TRIS/HCL PH 8.5, 2.1 M KCL, TEMPERATURE 295 K |
