5A38
Mutations in the Calponin homology domain of Alpha-Actinin-2 affect Actin binding and incorporation in muscle.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.300, 46.560, 70.160 |
| Unit cell angles | 73.81, 80.26, 75.38 |
Refinement procedure
| Resolution | 67.000 - 1.900 |
| R-factor | 0.19377 |
| Rwork | 0.192 |
| R-free | 0.23088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wku |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.324 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.650 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.070 | 0.390 |
| Number of reflections | 33050 | |
| <I/σ(I)> | 7.2 | 2.1 |
| Completeness [%] | 94.0 | 94 |
| Redundancy | 1.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 22% POLYETHYLENE GLYCOL 3350, pH 7.5 |
