5A2O

Crystal structure of the nitrate transporter NRT1.1 from Arabidopsis thaliana in complex with nitrate.

Replaces:  4CL5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-30
Detector	DECTRIS PILATUS 2M
Spacegroup name	P 21 21 21
Unit cell lengths	121.830, 123.590, 153.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.740 - 3.710
R-factor	0.2891
Rwork	0.287
R-free	0.32750
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.740	3.890
High resolution limit [Å]	3.710	3.710
Rmerge	0.090	0.530
Number of reflections	22013
<I/σ(I)>	19.3	2.1
Completeness [%]	87.6	87.6
Redundancy	3.7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	22 % PEG 400, 0.05 M SODIUM CITRATE PH 4.5, 0.07 M SODIUM CHLORIDE AND 1.5 % PEG 600 USING THE HANGING DROP VAPOR DIFFUSION TECHNIQUE AT 4 CELSIUS.