5A2E
Extracellular SRCR domains of human CD6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-31 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 161.480, 161.480, 93.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.930 - 3.150 |
| R-factor | 0.2545 |
| Rwork | 0.253 |
| R-free | 0.28310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1by2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.760 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.930 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.230 | 0.800 |
| Number of reflections | 12884 | |
| <I/σ(I)> | 7.2 | 2.2 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M AMMONIUM SULFATE, 0.3M SODIUM FORMATE, 0.1M SODIUM CACODYLATE, 3% W/V PGA-LM, 20% MPD, PH 6.5 |
