5A1M
Crystal structure of calcium-bound human adseverin domain A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 92.510, 92.510, 68.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.470 - 1.810 |
| R-factor | 0.1824 |
| Rwork | 0.182 |
| R-free | 0.18940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1RGI RESIDUES 271-364 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.110 | 0.890 |
| Number of reflections | 13860 | |
| <I/σ(I)> | 21.6 | 3.5 |
| Completeness [%] | 100.0 | 97 |
| Redundancy | 14.2 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30 % POLYETHYLENE GLYCOL 6000, 100 MM HEPES PH 7.0 |
