5A1L
Crystal structure of JmjC domain of human histone demethylase UTY with S21056a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.711, 110.500, 119.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.120 - 2.000 |
| R-factor | 0.18981 |
| Rwork | 0.188 |
| R-free | 0.22493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zli |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.337 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4I |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.310 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.190 | 0.890 |
| Number of reflections | 81121 | |
| <I/σ(I)> | 6.1 | 2.3 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG3350, 0.1M BIS-TRIS PH 6.5 |
