5A15
Crystal structure of the BTB domain of human KCTD16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-25 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.790, 69.370, 134.240 |
| Unit cell angles | 102.45, 95.72, 90.71 |
Refinement procedure
| Resolution | 34.650 - 2.760 |
| R-factor | 0.2185 |
| Rwork | 0.216 |
| R-free | 0.25860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4UES |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.550 | 2.830 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 50928 | |
| <I/σ(I)> | 13.7 | 2.1 |
| Completeness [%] | 97.9 | 98.2 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 21% PEG3350, 0.1M BIS-TRIS PH 6.0 |
