5A0R
Product peptide-bound structure of metalloprotease Zmp1 variant E143A from Clostridium difficile
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-09 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.060, 42.960, 119.210 |
| Unit cell angles | 90.00, 92.54, 90.00 |
Refinement procedure
| Resolution | 40.411 - 1.251 |
| R-factor | 0.1425 |
| Rwork | 0.141 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a0p |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.489 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (in) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 1.330 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 102218 | |
| <I/σ(I)> | 15.82 | 2.67 |
| Completeness [%] | 98.6 | 93.7 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 1.8 M AMMONIUM PHOSPHATE DIBASIC, 0.1 M TRIS PH 8.5 GROWN AT 293 K |
