5A05
Crystal structure of aldose-aldose oxidoreductase from Caulobacter crescentus complexed with maltotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.295, 151.321, 108.693 |
| Unit cell angles | 90.00, 108.66, 90.00 |
Refinement procedure
| Resolution | 48.745 - 1.897 |
| R-factor | 0.1535 |
| Rwork | 0.152 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.620 |
| Number of reflections | 239445 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 99.0 | 97.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MES PH 6.5, AMMONIUM SULPHATE, 2-PROPANOL |
