5A04
Crystal structure of aldose-aldose oxidoreductase from Caulobacter crescentus complexed with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.154, 153.516, 108.145 |
| Unit cell angles | 90.00, 109.35, 90.00 |
Refinement procedure
| Resolution | 47.591 - 1.698 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.600 |
| Number of reflections | 338561 | |
| <I/σ(I)> | 13 | 2 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | MES PH 6.5, AMMONIUM SULPHATE, DIOXANE |
