4ZOH
Crystal structure of glyceraldehyde oxidoreductase
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| 格子定数 [Å] | 143.431, 143.431, 235.509 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 分解能 | 41.400 - 2.200 |
| R因子 | 0.17998 |
| Rwork | 0.177 |
| R-free | 0.24231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ffv |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.020 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.280 |
| 分解能 [Å] (高) | 2.200 | 2.200 |
| Rmerge_l_obs | 0.394 | |
| 独立反射数 | 73074 | |
| <I/σ(I)> | 36.7 | 6.9 |
| 完全性 [%] | 100.0 | 100 |
| 冗長性 | 12.1 | 12.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 MNa-acetate, 0.2M CH3COONH4, 15% (w/v) PEG 4000 |
