4V7B
Visualization of two tRNAs trapped in transit during EF-G-mediated translocation
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | blot for 5-10 seconds before plunging |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | KODAK SO-163 FILM |
Number of Images | 308 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 6.8 |
Software | UCSF Chimera,SPIDER |
Symmetry | C1 |