4V2E

FLRT3 LRR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PIXEL
Spacegroup name	P 1 21 1
Unit cell lengths	59.810, 61.970, 92.970
Unit cell angles	90.00, 106.38, 90.00

Refinement procedure

Resolution	57.380 - 2.500
R-factor	0.3451
Rwork	0.345
R-free	0.35140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2d
RMSD bond length	0.008
RMSD bond angle	0.900
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.460
Number of reflections	63975
<I/σ(I)>	3.1	0.98
Completeness [%]	90.4	89.2
Redundancy	3.1	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1