Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 436.250, 286.920, 303.840 |
| 格子定数 [度] | 90.00, 98.90, 90.00 |
精密化法
| 残基 | 49.690 - 3.000 |
| R因子 | 0.2088 |
| Rwork | 0.208 |
| R-free | 0.25460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.012 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.616 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Quality characteristics
| Overall | Inner shell | Outer shell | |
| 分解能 [Å] (低) | 49.690 | 3.100 | |
| 分解能 [Å] (高) | 3.000 | 40.000 | 3.000 |
| Rmerge_l_obs | 0.341 | 0.062 | 3.527 |
| Rmeas | 0.361 | 0.072 | 3.725 |
| Total number of observations | 13621629 | ||
| 独立反射数 | 1451516 | 280 | 134798 |
| <I/σ(I)> | 7.13 | 11.84 | 0.88 |
| 完全性 [%] | 98.8 | 41.2 | 98.2 |
| 冗長性 | 9.37 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
