4Q46
The second structure of Influenza B PB2 cap-binding domain complex with GDP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 43.074, 88.543, 52.151 |
| 格子定数 [度] | 90.00, 105.18, 90.00 |
精密化法
| 残基 | 24.570 - 1.800 |
| R因子 | 0.17301 |
| Rwork | 0.170 |
| R-free | 0.23065 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oro |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.025 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.170 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 24.570 | 1.830 |
| 分解能 [Å] (高) | 1.800 | 1.800 |
| Rmerge_l_obs | 0.077 | 0.217 |
| 独立反射数 | 33162 | |
| 完全性 [%] | 94.6 | 98.2 |
| 冗長性 | 5.5 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2M Sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
