4ORC
Crystal structure of mammalian calcineurin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-12 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 110.200, 110.200, 282.488 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.538 - 2.700 |
| R-factor | 0.2176 |
| Rwork | 0.215 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aui |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.205 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.098 | 0.615 |
| Number of reflections | 28655 | |
| <I/σ(I)> | 23.9 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 294 | 100mM MES, pH 6.1, 10% PEG3350, 0.2M CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
