4OEF
Crystal Structure Analysis of FGF2-Disaccharide (S6I2) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-22 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 |
Unit cell lengths | 30.417, 33.083, 35.608 |
Unit cell angles | 65.52, 72.60, 77.35 |
Refinement procedure
Resolution | 24.304 - 1.800 |
R-factor | 0.1744 |
Rwork | 0.169 |
R-free | 0.22010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1bfc |
RMSD bond length | 0.006 |
RMSD bond angle | 1.197 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.046 | 0.274 |
Number of reflections | 10466 | |
<I/σ(I)> | 36.2 | 6.7 |
Completeness [%] | 94.2 | 91 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 299 | 40% polyethylene glycol 600 and 100 mM Na2HPO4/citric acid, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 299K |