4IH4
Crystal structure of Arabidopsis DWARF14 orthologue, AtD14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 184.856, 44.629, 160.967 |
Unit cell angles | 90.00, 121.07, 90.00 |
Refinement procedure
Resolution | 30.000 - 3.500 |
Rwork | 0.279 |
R-free | 0.33630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ih1 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.620 |
High resolution limit [Å] | 3.500 | 7.500 | 3.500 |
Rmerge | 0.204 | 0.065 | 0.557 |
Number of reflections | 12471 | ||
<I/σ(I)> | 4 | ||
Completeness [%] | 83.8 | 78.6 | 85.6 |
Redundancy | 4.1 | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 298 | PEG4000, MgCl2, pH 8.5, vapor diffusion, temperature 298K |