4F7H

The crystal structure of kindlin-2 pleckstrin homology domain in free form

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-07
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
格子定数 [Å]	58.474, 58.474, 92.249
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.237 - 1.900
R因子	0.1964
Rwork	0.195
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ys3
結合長の平均二乗偏差(RMSD) [Å]	0.013
結合角の平均二乗偏差(RMSD) [度]	1.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	29.237	1.940
分解能 [Å] (高)	1.900	1.900
独立反射数	13245
完全性 [%]	99.9	100
冗長性	9.4	6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	1.4 M ammonium tartrate dibasic, 0.1 M tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K