4EBQ
Fab structure of anti-Vaccinia virus D8L antigen mouse IgG2a LA5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.100002 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.190, 93.360, 135.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.120 - 1.600 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nsn |
RMSD bond length | 0.012 |
RMSD bond angle | 1.467 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 135.960 | 31.120 | 1.690 |
High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
Rmerge | 0.026 | 0.323 | |
Total number of observations | 11799 | 42438 | |
Number of reflections | 59711 | ||
<I/σ(I)> | 17.7 | 21.6 | 2.4 |
Completeness [%] | 96.6 | 97.5 | 93.8 |
Redundancy | 5.5 | 5.6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 10% w/v PEG3000, 100 mM phosphate/citrate, 200 mM sodium chloride, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |