4DNC

Crystal structure of human MOF in complex with MSL1

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98159
Spacegroup name	P 1 21 1
格子定数 [Å]	64.801, 111.518, 66.751
格子定数 [度]	90.00, 111.05, 90.00

精密化法

残基	40.994 - 2.050
R因子	0.2123
Rwork	0.208
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.007
結合角の平均二乗偏差(RMSD) [度]	1.064
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Quality characteristics

	Overall
分解能 [Å] (低)	100.000
分解能 [Å] (高)	2.050
独立反射数	54362

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	7	277	1.6 M Li2SO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K