4AK0
Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-01-06 |
Detector | MARRESEARCH |
Spacegroup name | I 21 3 |
Unit cell lengths | 77.800, 77.800, 77.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.050 - 2.280 |
R-factor | 0.17263 |
Rwork | 0.166 |
R-free | 0.23165 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b2f |
RMSD bond length | 0.018 |
RMSD bond angle | 1.802 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.000 | 2.420 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.120 | 0.380 |
Number of reflections | 3406 | |
<I/σ(I)> | 18.4 | 7 |
Completeness [%] | 91.9 | 100 |
Redundancy | 12.1 | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.8 | pH 7.8 |