4ZZK
Crystal structure of truncated FlgD (monoclinic form) from the human pathogen Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95372 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 77.420, 34.220, 131.640 |
Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
Resolution | 38.390 - 2.750 |
R-factor | 0.22599 |
Rwork | 0.221 |
R-free | 0.27487 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zzf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.490 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.090 | 2.880 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.077 | |
Number of reflections | 18375 | |
<I/σ(I)> | 14.4 | 2.8 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | SPG,PEG 1500 |