4ZZK
Crystal structure of truncated FlgD (monoclinic form) from the human pathogen Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 77.420, 34.220, 131.640 |
| Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
| Resolution | 38.390 - 2.750 |
| R-factor | 0.22599 |
| Rwork | 0.221 |
| R-free | 0.27487 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zzf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.490 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.090 | 2.880 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.077 | |
| Number of reflections | 18375 | |
| <I/σ(I)> | 14.4 | 2.8 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | SPG,PEG 1500 |
