4ZZF
Crystal structure of truncated FlgD (tetragonal form) from the human pathogen Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.99988 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 76.418, 76.418, 145.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.380 - 2.167 |
| R-factor | 0.1984 |
| Rwork | 0.197 |
| R-free | 0.23380 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.108 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.380 | 2.280 |
| High resolution limit [Å] | 2.167 | 2.167 |
| Rmerge | 0.059 | 0.666 |
| Number of reflections | 11714 | |
| <I/σ(I)> | 20.5 | 2.1 |
| Completeness [%] | 98.5 | 90 |
| Redundancy | 6.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Sodium acetate, Bis-Tris propane, PEG 3350 |
