4ZZ6
Structure of the complex of type 1 ribosome inactivating protein from Momordica balsamina with a nucleotide, cytidine triphosphate at 2.0A resolution
Replaces: 4K6SExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-11-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 |
| Unit cell lengths | 129.964, 129.964, 40.495 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.130 - 2.000 |
| R-factor | 0.16339 |
| Rwork | 0.163 |
| R-free | 0.18871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s9q |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.130 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 16425 | |
| <I/σ(I)> | 47.8 | 7.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate |
