4ZYM
Structural implications of homo-pyrimidine base pairs on the parallel-stranded d(GAY) motif.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.954, 29.354, 74.731 |
| Unit cell angles | 90.00, 112.47, 90.00 |
Refinement procedure
| Resolution | 69.057 - 2.530 |
| R-factor | 0.2305 |
| Rwork | 0.230 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rim |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.725 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.057 | 2.620 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.103 | 0.286 |
| Number of reflections | 4342 | |
| <I/σ(I)> | 4.5 | 1.9 |
| Completeness [%] | 98.7 | 95 |
| Redundancy | 2.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 100 mM Magnesium chloride, 5 % PEG400, 30 mM Sodium Cacodilate, 8 mM Cobalt hexamine. |
